Figure 7.

Anti-IL-10 antibody abolished the effect of  IVIG on the suppression of IL-12p70 production in BMDC stimulated with LPS. Cells were stimulated with LPS (1 μg/mL) in the presence or absence of  IVIG (5 mg/mL) and the anti-IL-10 or its control antibody for 18 h. IL-12p70 production in the culture supernatant was measured using the ELISA kit. Results were expressed as mean ± SEM (n = 3). ^## P < 0.01, significantly different from the medium alone (without LPS stimulation, Student's t-test); **P < 0.01, significantly different from the Control (LPS stimulation without IVIG, Dunnett's multiple comparison test). ND, cytokine concentrations were under the lower limit values of the standard ELISA curves. Three independent experiments were conducted and representative results were shown.