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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cytokine. 2013 May 11;63(2):151–165. doi: 10.1016/j.cyto.2013.04.022

Figure 1. PolA2EN cells are susceptible to C. trachomatis serovar D infection, and chlamydiali infection does not significantly compromise the polarized monolayer.

Figure 1

(a) Using the rocking protocol, infection rates of C. trachomatis in polA2EN cells never exceeded approximately 25% no matter the dose of chlamydial inoculum used. (b) Using the centrifugation protocol for C. trachomatis infection, polA2EN cells were capable of being infected at an infection rate of >95% when 1.0μL chlamydial inoculum was used. No significant cell lysis was observed in infected polA2EN cells. Infection rates shown are mean levels ± the standard deviation (SD) from 3 independent experiments each performed in triplicate. (c) TEER values were not statistically significant between mock infected cells and C. trachomatis infected cells up to 4 days post-infection. Data points represent mean values ±SD pooled from 3 independent experiments each performed in triplicate.