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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cytokine. 2013 May 11;63(2):151–165. doi: 10.1016/j.cyto.2013.04.022

Figure 11. Supernatants from C. trachomatis infected polA2EN cells do not amplify the pro-inflammatory cytokine response in non-infected cells.

Figure 11

PolA2EN cells were exposed to filtered supernatants from mock infected and C. trachomatis serovar D infected polA2EN cells for 4 hours. Supernatants were removed, and fresh medium was incubated with cells for 12 hours. Unstimulated polA2EN cells were utilized as a control. Apical and basolateral supernatants were collected and subjected to cytometric bead array analyses for (a) IL6, (b) CXCL8, (c) IP10, and (d) RANTES. Cytokine values represent mean values ± SD from 3 independent experiments each performed in triplicate. Statistical analyses between parameters were performed using a One Way ANOVA.