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TMR dimers as allosteric probes. The absorbance (A) and steady-state fluorescence emission (B) of a sample of EL242C-TMR with 4.3 TMR per ring is examined in the absence (green) and presence (orange) of 500 µM ATP. In the absence of ATP, the enzyme is in the T state, indicated by the population of dimers. On transition to the R state, the dimers dissociate into monomers, causing a change in the peak absorbance and increase in the fluorescence.
