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. 2013 Jun 19;110(27):11175–11180. doi: 10.1073/pnas.1301849110

Fig. 1.

Fig. 1.

MIG-13 in C. elegans Q-neuroblast migration. (A) Q-neuroblast asymmetric divisions generate three neurons and two apoptotic cells (X in black). QL produces PQR, PVM, and SDQL, and QR produces AQR, AVM, and SDQR. (B) QR descendants (QR.x), AQR (cyan circle) and AVM (blue), migrate anteriorly, and the QL descendant (QL.x), PQR (cyan), migrates posteriorly. Lin-39 and mab-5 regulate Q-cell migration (two gray double arrow lines). (C) Genetic screens using zdIs5[Pmec-4::GFP] and casIs35[Pgcy-32::mCherry] for mutations defective in QR.x anterior migration. AVM and PVM (green) or AQR and PQR (red) from Q-cell lineages were marked by cell type-specific promoters. (D) C. elegans MIG-13 and mouse Lrp12 protein domains. The amino acid changes or deletion in mig-13 mutant alleles (cas14, cas15, cas64, cas65) are indicated. (E) Quantifications of AVM position of mig-13 (mu225) and the rescue experiments. Genetic backgrounds were indicated on the Upper Left. n = 60–100 from a single experiment. Statistical analysis is shown in Fig. S3B. (F) Still images of muIs62 [MIG-13::GFP] (green) during WT QR.x migration. Q-cell plasma membrane and chromosomes are imaged by mCherry. Merged images are shown at Bottom. The cell name is adjacent. The anterior of the cell is on the Left. Time is in minutes. (Scale bar, 5 µm.) (G) Percentage of Q.ap cells expressing mig-13 in lin-39 (n1760), mab-5 (e2088), or mig-14 (mu71) mutants. n = 12–30 from a single experiment. ***P < 0.001 by Student t test.