Skip to main content
. 2013 Jun 19;110(27):11175–11180. doi: 10.1073/pnas.1301849110

Fig. 4.

Fig. 4.

LIN-39 regulates the QR.x anterior polarity by promoting mig-13 expression. (A) Still images show QR.ap migration in lin-39(n1760) mutants. COR-1::GFP in green and Q-cell plasma membrane marked with mCherry in red. The long yellow arrow shows the anterior migration of WT QR.ap. The short white arrows show defects of QR.ap polarity in lin-39 (n1760) mutants. Time is in minutes. (Scale bar, 5 µm.) (B) ChIP-seq data show that LIN-39 binds to three sites (A–C, blue, Upper) in the mig-13 promoter. Input (the negative control in ChIP-Seq) is in red, middle. The mig-13 gene model is in black (Bottom). (C) AQR and AVM positions in WT, mig-13 (mu225), and mig-13 rescue experiments with LIN-39 binding site deletion. The position and range of binding site A, B, C, and motif (consensus DNA motif for LIN-39 target genes) are in the text. ***, nonrescue, P < 0.001; *, partial rescue, P < 0.01 by Student t test. (D) AQR (Left) and AVM (Center) positions in WT, lin-39 (n1760), and mig-13 rescue backgrounds. Schematics (Right) of AQR and AVM positions. *P < 0.01 by Student t test.