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GUS activity in S. coelicolor derivatives containing psp promoter-gusA fusions in the absence or presence of either pspX or pspR transcribed from PermE*. (A) Spectrophotometric assay using p-nitrophenyl-β-d-glucoronide as substrate. Miller units are shown per milligram of protein present in cell lysate. Error bars depict SD between three biological replicates. (B) Chromogenic assay on R2 agar containing the substrate 5-bromo-4-chloro-3-indolyl-β-d-glucoronide.
