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31P-NMR analysis of DhpJ activity with a 1:1.2 mixture of monomethylated and unmethylated l- Leu-l-Ala(P). (A) Reaction scheme of DhpJ activity. (B) 31P-NMR spectrum of the a solution of l-Leu-l-Ala(POMe) and l-Leu-l-Ala(P). (C) 31P-NMR spectrum taken 6 h after addition of Fe(II)-reconstituted DhpJ, α-KG, O2, and l-ascorbic acid. MAP was presumably the side product of l-Leu-ΔAla(POMe) hydrolysis. The phosphate peak (between 2 and 4 ppm) was omitted for clarity.
