Table 1.
Data collection and refinement statistics
| Dataset |
 ;Ψ27-AUG
|
 ;Ψ27-AUA
|
| Data collection | ||
| Space group | P41212 | P41212 |
| Cell dimensions | ||
| a, b, c (Å) | 400.6, 400.6, 176.2 | 402.4, 402.4, 175.6 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 80.1–3.3 (3.38–3.30) | 97.6–3.3 (3.38–3.30) |
| Rmerge | 22.5 (133.7) | 19.8 (113.3) |
| I/σI | 6.35 (1.19)* | 5.50 (1.25)* |
| CC1/2 | 0.99 (0.41) | 0.99 (0.49) |
| Completeness (%) | 97.4 (95.0) | 94.7 (93.5) |
| Redundancy | 3.3 (3.2) | 2.6 (2.5) |
| Refinement | ||
| No. reflections | 207,954 | 230,910 |
| Rwork/Rfree | 18.8/22.2 | 19.5/23.6 |
| No. atoms | 52,227 | 52,187 |
| RNA | 32,766 | 32,726 |
| Protein | 19,231 | 19,231 |
| Ion | 188 | 188 |
| Paromomycin | 42 | 42 |
| B-factor | 80.1 | 87.4 |
| r.m.s. deviations | ||
| Bond lengths (Å) | 0.008 | 0.008 |
| Bond angles (°) | 1.36 | 1.35 |
Parentheses indicate highest-resolution shell.
*
I/σI = 2 at 3.48 Å for both structures.