FIG. 2.

Tregs are immunosuppressive in healthy controls (HC), but not AITD. (A) Representative T-cell proliferation. CD4⁺ T cells from one HC subject, one GD patient, and one HT patient were activated at various ratios of Teff to Treg. Effector cell density was maintained at 20,000 cells/well, and Treg populations were titered from 10,000 to 2500. [³H]-thymidine was added for the final 15–18 hours of culture, and proliferation was measured by scintillation counting. (B, C) Percentage proliferation of activated Teff cells when cocultured with various concentrations of Tregs. (B) A 20,000:10,000 ratio of Teff to Treg. (C) A 20,000:5000 ratio of Teff to Treg. Horizontal bars show the mean with SEM. A one-way ANOVA Bonferonni test was used for multiple group comparisons in (B) and (C).