Table 3.
Cytokine Production in the Presence and Absence of Autoimmune Thyroid Disease
| Presence of AITD | 20,000:0 | 20,000:10,000 | p | |
|---|---|---|---|---|
| TGF-β | − | 904±374.0 | 839.5±355.4 | 0.34 |
| + | 947.4±105.5 | 908.6±99.41 | 0.36 | |
| IL-10 | − | 149.4±50.65 | 181±63.07a | 0.44 |
| + | 94.92±27.27 | 131.4±29.66a | 0.1253 | |
| IL-4 | − | 38.68±24.37 | 43.73±25.47 | 0.3429 |
| + | 21.02±5.65 | 24.14±5.46 | 0.3962 | |
| IL-17 | − | 331.8±142.7 | 598±276.6 | 0.1833 |
| + | 290.6±80.83 | 912.8±254.5 | 0.0228* | |
| IL-5 | − | 21.08±14.38 | 38.22±13.32 | 0.2 |
| + | 185.2±76.54 | 299.7±105.3 | 0.2863 | |
| IL-2 | − | 1002±683.4 | 298.4±217.7 | 0.3429 |
| + | 1510±418.9 | 524.5±222.2 | 0.0228* | |
| IFN-γ | − | 34,720±18,436 | 57,694±31,612 | 0.3429 |
| + | 34,046±15,544 | 23,754±10,662 | 0.4361 |
Conditioned media from Teff cells cultured alone and cocultured with Tregs at 20,000:10,000 were evaluated at 96 hours. Samples were obtained from four HC and 14 patients with AITD. Data represent mean and SEM (pg/mL); p values represent a comparison of 20,000:0 and 20,000:10,000.
a
Significant decrease in IL-10 production in coculture of AITD versus HC.
*
p value<0.05.
TGF, transforming growth factor; IL, interleukin; IFN, interferon.