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. 2013 Aug 10;19(5):482–496. doi: 10.1089/ars.2011.4421

FIG. 10.

FIG. 10.

ROS-dependent reciprocal regulation between Prx I and Nrf2. (A) Prx I deficiency caused increased expression levels of Nrf2 and HO-1 and a decrease in Keap1 in 3T3-MEFs. The data are representative of at least three different experiments and presented as mean±SEM. *p<0.05, **p<0.01 compared with WT 3T3-MEFs. (B, C) Effect of Prx I knockdown on Nrf2, keap1, and HO-1 protein levels in A549 (B) and NCI-H358 cells (C). (D) A549 cells were stably transfected with human Prx I vectors and cell lysates derived from two established independent stable clones (#2 and #19); they were subjected to immunoblotting with anti-Prx I, Nrf2, Keap1, and HO-1. Immunoblotting with an anti-HA antibody showed exogenously overexpressed Prx I. (E) Effect of combined treatment with siPrx I and NAC on protein levels of Nrf2, Keap1, and HO-1 in A549 cells. (F) A549 cells were transfected with siNrf2 and subjected to Western blotting analysis against Nrf2, pERK, ERK, and cyclin D1. The data are representative of at least three different experiments.