FIG. 2.

Prx I is up-regulated by an oncogenic K-ras via the Nrf2 transcription factor. (A) H&E and anti-Prx I were stained in lung tissues of non-Tg (NTg) and K-ras^G12D-driven lung cancer model mice (K-ras^G12D-Tg) at 3 months. Tumors are indicated by black arrows on the lung surface (Scale bars, 50 μm). (B, C) Expression levels of K-ras and Prx I in siK-ras-transfected A549 and NCI-H358 cells were analyzed by Western blotting and quantitative PCR (qPCR) analyses. GAPDH was used as a loading control. The data are representative of at least three different experiments and presented as mean±SEM. ^*p<0.05, ^**p<0.01, and ^***p<0.001 compared with the cells transfected with scrambled siRNA. (D) Western blots were performed for Nrf2, Keap1, and HO-1 in siK-ras-transfected A549 and NCI-H358 cells. The data are representative of at least three different experiments. HO-1, heme oxygenase-1; Tg, transgenic. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars