FIG. 4.
Deficiency of Prx I impaired cell proliferation and anchorage-independent growth. (A) Generation of stable cell lines expressing K-rasG12D in WT and Prx I−/− 3T3-MEFs. (B) Cellular proliferation effects of K-rasG12D 3T3-MEFs with Prx I inactivation were measured by CCK-8 assay at the indicated time. The data are representative of at least three different experiments and presented as mean±SEM. *p<0.05, ***p<0.001 compared with WT-K-rasG12D 3T3-MEFs. (C) Anchorage-independent growth assay was performed in 3T3-MEFs transfected with transiently or stably expressing K-rasG12D. The diameters of the colonies were indicated by black (100–150 μm) or white (>150 μm) arrows. (D) The number of colonies was determined by counting duplicated plates. The diameters of the colonies were 50–100 μm, 100–150 μm, and >150 μm. CCK-8, cell counting kit-8; MEFs, mouse embryonic fibroblasts.