Topical application of NC1 did not promote wound healing. A 1.0 cm2
(1 × 1 cm) square full-thickness excision wounds were made on
the mid-back of 8- to 10-week-old athymic nude mice and purified NC1 domain
(30 g) (n = 10 mice) or vehicle (VE) (n = 10 mice) were applied
topically once on day 0. (a) Representative days 0, 7, 12, and 14 wounds are
shown for wounds treated with NC1 or vehicle alone (VE). (b) Mean ± SD
wound size measurements at days 0, 7, 9, 12, and 14 after wounding treated with NC1
(n = 10 mice for each group).