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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Gene Expr Patterns. 2013 Apr 29;13(7):225–239. doi: 10.1016/j.gep.2013.04.003

Fig. 4. IFITM3 localization to the anterior region (EHF-14-s stages; ~E7.75-9.0).

Fig. 4

A: EHF stage. Large panel is a magnification of area boxed within inset. IFITM3 localized to the apical surface of some endoderm at the embryonic-extraembryonic junction of the anterior embryo (black arrows) and to the apical surface of the anterior-most neurectoderm (ne; red arrow) of the headfolds (hf). B: 2-s stage. Large panel is a magnification of area boxed within inset. IFITM3 continued to localize to the apical surface of some anterior embryonic endoderm (black arrow) near the extraembryonic visceral endoderm (xve) and anterior-most neurectoderm (red arrow). C: 4-s stage. IFITM3 localized to the apical surface of the majority of endoderm overlying the heart (ht; black arrows), anterior surface ectoderm (black arrowheads), and foregut (fg; highlighted by black box in larger panel and magnified within inset, with red arrowheads). D: 5-s stage. IFITM3 continued to localize to the apical surface of the endoderm overlying the heart (black arrow) and some neurectoderm (red arrow). In addition, IFITM3 localized as a cytoplasmic spot to some anterior mesenchymal cells (highlighted by black boxes in larger panel and magnified within insets). E: 7-s stage. IFITM3 localized to the apical surface of endoderm overlying the heart (black arrows), endoderm at the foregut opening, and surface ectoderm (black arrowhead). F: 14-s stage. IFITM3 persisted in the apical surface of the ventral foregut (red arrowhead outlined in black). G: 10-s stage. Large panels are magnifications of boxed areas within upper right insets. IFITM3 localized to the apical surface of anterior surface ectoderm posterior to (G1; black arrow), but not overlying (G2), the branchial arches (ba). IFITM3 localized as a cytoplasmic spot to some mesenchymal cells within the branchial arches (highlighted by black box in larger panel and magnified within lower right panel). H: 10-s stage. IFITM3 localized as a cytoplasmic spot to some lateral mesoderm adjacent to the somites (s). Area highlighted by black box is magnified within inset. Other abbreviations: da, dorsal aortae. Scale bar in A: 10 μm (A, B); 39.7 μm (A inset); 62.2 μm (B inset); 25.0 μm (C-E, G, H); 8.5 μm (C inset); 10.8 μm (D left inset); 11.3 μm (D right inset); 30.1 μm (F); 113.0 μm (G1 inset); 124.4 μm (G2 upper inset); 4.5 μm (G2 lower inset); 8.0 μm (H inset).