Fig. 2.
(A) Hepatic 4-HNE concentrations in lean and obese (ob/ob) mice fed 0%, 0.5% or 1% GTE for 6 weeks (n=12–13 mice/group; means±S.E.). Liver homogenates were prepared in RIPA buffer, and HNE was measured by ELISA. (B) Hepatic NADPH oxidase activity in lean and obese (ob/ob) mice fed GTE at 0%, 0.5% and 1% for 6 weeks (n=12–13 mice/group; means±S.E.). NADPH oxidase from liver homogenates was evaluated by lucigenin-enhanced chemiluminescence following incubation with lucigenin and NADPH. Means not sharing a common superscript are significantly different, P<.05.