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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Integr Biol (Camb). 2013 Jan;5(1):151–158. doi: 10.1039/c2ib20119d

Figure 1.

Figure 1

Preparation of recombinant TAT vaults. A. A schematic representation of a TAT-vault and a CP-vault packaged with mCherry-INT fusion proteins. CP peptides (MAGCGCPCGCGA, in yellow) were covalently fused to the N-terminus of the major vault protein and, following expression and self assembly, they are displayed on the inside of recombinant vault nanoparticles. TAT peptides (GRKKRRQRRRAHQ, in blue) were covalently fused to the C-terminus of the major vault protein and, following expression and self assembly, they are displayed on the outside of recombinant vault nanoparticles. mCherry-INT fusion proteins are non-covalently bind to the INT binding side located on the inside of fully assembled vault nanoparticles. B. TEM image showing the negatively stained purified TAT vaults. C. Coomassie stain of purified recombinant TAT vaults containing mCherry-INT fusion proteins. The MVP-TAT (∼100kDa) and mCherry-INT (∼45kDa) proteins are indicated on the right and the positions of the molecular weight standards are indicated at the left.