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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Integr Biol (Camb). 2013 Jan;5(1):151–158. doi: 10.1039/c2ib20119d

Figure 2.

Figure 2

The uptake efficiency of TAT vaults is significantly higher than that of the control vaults in Hela cells. A. Western analysis shows an enhanced uptake of TAT vaults in Hela cells. The indicated amounts of CP/mCherry (control) or TAT/mCherry vaults were incubated with 500,000 Hela cells for 16 hours. Hela cells were then washed three times with the cold PBS solution. Whole cell lysates were separated on an SDS PAGE gel and probed with anti-MVP antibody. B. Confocal images of live Hela cells (500, 000 cells/dish) incubated with 10 μg of CP/mCherry (left) or TAT/mCherry (right) vaults for 16 hours. The mCherry channel (in red) is overlaid on top of the bright field view. C. Western analysis confirming similar patterns of cell surface binding to both Hela and U2OS cells. A proportional correlation between the amount of TAT/mCherry vaults added to the dish and the amount of TAT/mCherry vaults recovered from Hela and U2OS cells was observed.