Figure 5.
Mitotic division of differentiated ICC. BrdU incorporation demonstrates that differentiated Kit+-ICC undergo mitotic division. (A) Kit+-ICC networks in a P10 wildtype mouse (arrow). (B) BrdU incorporation of cells at the myenteric plexus (arrow). (C). Double labeling of Kit revealed cellular co-localization with BrdU labeling (arrow). (D) Higher magnification demonstrating the incorporation of BrdU into differentiated Kit+-ICC. Note the presence of anastomosing projections, a morphological feature of mature ICC (arrow). Scale bar = 50 μm in C for panels A–C. Scale bar in D = 25 μm.