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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Cell Metab. 2008 May;7(5):434–444. doi: 10.1016/j.cmet.2008.02.010

Figure 3. Neuronal TORC Expression Rescues Sensitivity of TORC25-3 Flies to Starvation and Oxidative Stress.

Figure 3

(A) Relative survival in response to starvation (top) or paraquat feeding (bottom) of WT, TORC25-3, TORC25-3,ELAV-GAL4 control, and TORC25-3,ELAV-GA-L4,UAS-TORC rescued flies. n = 100; p < 0.05.

(B) Top: relative lipid content of TORC25-3,ELAV-GAL4 control and TORC25-3,ELAV-GAL4,UAS-TORC rescued flies. n = 6 flies per group; *p < 0.05. Bottom: immunoblot of FOXO and 4E-BP proteins in TORC mutant TORC25-3,ELAV-GAL4 and TORC25-3,ELAV-GAL4,UAS-TORC rescued flies under fed (F), starved (S), or refed (R) conditions.

(C) CRE-luc activity in TORC25-3,ELAV-GAL4 control and TORC25-3,ELAV-GAL4,UAS-TORC rescued flies. n = 6; *p < 0.05.

(D) qPCR analysis of TORC-regulated genes from head mRNAs of TORC25-3,ELAV-GAL4 control and TORC25-3,ELAV-GAL4,UAS-TORC rescued flies under fasting conditions. Rescue genotype: ELAV-GAL4, w1118/Y;UAS-TORC/+;TORC25-3. Control genotype: ELAV-GAL4, w1118/Y;TORC25-3. Wild-type: w1118.

Data are given as means ± SD.