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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Cell Metab. 2008 May;7(5):434–444. doi: 10.1016/j.cmet.2008.02.010

Figure 4. The Insulin Signaling Pathway Regulates TORC Activity in Drosophila.

Figure 4

(A) Immunoblot of phospho-TORC and total TORC protein in fasted or refed flies. Hours after refeeding are indicated. Amounts of phospho-AKT are also shown.

(B) Immunoblots showing amounts of phospho-TORC and total TORC in WT and insulin-producing cell (IPC)-ablated flies (top) and chico mutant flies (bottom) under fasted or refed conditions. Loss of insulin-like peptide (ilp) gene expression in IPC-ablated flies was confirmed by qPCR analysis (data not shown).

(C) Comparison of CRE-luc reporter activity in WT and chico mutant flies under fed or fasted conditions. n = 13 per group; p < 0.05.

(D) qPCR analysis showing relative expression of TORC-regulated genes in WT and IPC-ablated flies.

(E) qPCR analysis of ilp2, ilp3, and ilp5 gene expression in WT and TORC25-3 flies fed ad libitum.

(F) Effect of IPC ablation on starvation sensitivity in TORC25-3 flies.

Data are given as means ± SD.