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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Cell Metab. 2008 May;7(5):434–444. doi: 10.1016/j.cmet.2008.02.010

Figure 6. TORC Acts Downstream of the Serine/Threonine Kinase AKT to Regulate CREB Activity.

Figure 6

(A and B) Effect of TORC overexpression in postmitotic cells of the eye imaginal disc shown relative to control.

(C) Effect of depleting TORC via RNAi-mediated knockdown on eye morphology in TORC-overexpressing flies.

(D and E) Effect of CrebB disruption, either via mutation of the CrebB gene (CrebBS162; [D]) or in flies with a chromosomal deficiency (Df(1)N19; [E]), on eye phenotype in TORC transgenic flies.

(F) Eye morphology in TORC transgenic flies with reduced AKT (AKT RNAi) expression.

(Da–Fa) Effect of each disruption (CrebB, Df(1)N19, and AKT RNAi) on eye phenotype in the absence of TORC overexpression.

(G–Gb) Effect of PP2B overexpression alone (G), in TORC mutant flies (Ga), and in TORC-overexpressing flies (Gb).

(H and Ha) Effect of mutation of FOXO (FOXO21/FOXO25) on the TORC overexpression phenotype (Ha) compared to TORC overexpression alone (H).

Genotypes are GMR-GAL4/+;TM3/+ (A), GMR-GAL4/+;2X UAS-TORC/+ (B), GMR-GAL4/UAS-TORC RNAi;2X UAS-TORC/+ (C) CrebBS162/+;GMR-GAL4/+;2X UAS-TORC/+ (D), CrebBS162/+;GMR-GAL4/+ (Da), Df(1)N19/+; GMR-GAL4/+;2X UAS-TORC/+ (E), Df(1)N19/+;GMR-GAL4/+ (Ea), GMR-GAL4/UAS-AKT RNAi;2X UAS-TORC/+ (F), GMR-GAL4/UAS-AKT RNAi (Fa), GMR-GAL4/UAS-Pp2b-14Dact/+ (G), GMR-GAL4/UAS-Pp2b-14Dact;TORC25-3 (Ga), GMR-GAL4/UAS-Pp2b-14Dact;UAS-TORC/+ (Gb), GMR-GAL4/2X UAS-TORC (H), and GMR-GAL4/2X UAS-TORC;FOXO21/FOXO25 (Ha).