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TUNEL staining in wild-type versus IL6−/− mouse retinas harvested 3 days after detachment. (A, B) Wild-type mice. (C, D) IL-6−/− mice. (A, C) Fluorescein isothiocyanate (FITC) fluorescence of TUNEL-positive cells. (B, D) Propidium iodide (PI) fluorescence of all nuclei. (E) Graph summarizing TUNEL staining of wild-type and IL-6−/− mouse retinas 3 days after detachment. Results are mean ± SEM. *P = 0.002; IL-6−/− mice compared with wild-type mice.
