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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Nat Immunol. 2012 Jun 17;13(8):729–736. doi: 10.1038/ni.2352

Figure 3. The expression of M cell-markers in Spib−/− mice.

Figure 3

(a) Whole mount GP2 staining of PPs from Spib−/− and wild-type mice was performed. GP2 was visualized with Alexa 488 anti-rat IgG (green). F-actin was stained with Alexa 647 Phalloidin (blue). Dotted lines depict the position of FAE. Scale bar: 80 μm. (b) Expression of CCL9 and Marcksl1 in mouse FAE was analyzed by immunohistochemistry. Scale bar: 50 μm. (c) Comparison of M-cell marker expression in FAE and VE between wild-type and Spib−/− mice by qPCR analysis. Data represent fold change compared to the normalized value of expression of each transcript in villous epithelial cells from wild-type mice. All samples were normalized to the expression level of GAPDH. Data are representative of three independent experiments (error bars, s.d.). *, P<0.01.