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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Nat Immunol. 2012 Jun 17;13(8):729–736. doi: 10.1038/ni.2352

Figure 4. RANKL-induced M-cell differentiation in Spib−/− mice.

Figure 4

(a) Small intestines were dissected from wild-type and Spib−/− mice after 3 days of RANKL-treatment, and subjected to immunohistochemistry. The upper three panels show the immunostaining of M-cell markers GP2, CCL9 and Marcksl1 in wild-type mice, and the lower three panels show those in Spib−/− mice. Scale bar: 50 μm. (b) Quantitative analysis of M-cell marker expression by qPCR. Data represent fold change compared to the normalized value of expression of each transcript in villous epithelial cells from untreated wild-type mice. All samples were normalized to the expression level of GAPDH. Data are representative of two independent experiments (error bars, s.d.).