Fig. 6. F1126E does not affect deactivation time constants of the channel.

Sample wild-type (A) and E1126E (B) tail currents were measured in 10 mM Ba²⁺ and 10 mM Ca²⁺ as indicated. Tail currents were elicited by repolarizing steps to −20 mV following 50 msec steps to +50 mV. Tail currents measured in Ca²⁺ were normalized to those measured in Ba²⁺ from the same cell. (C) To quantify deactivation time constants (τ) at the indicated voltages, raw data were fitted with a single exponential equation. Average τ at the indicated repolarization potentials are shown for wild-type (n = 8) and F1126E (n = 5) in Ba²⁺ and Ca²⁺.