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. 2012 Apr 24;53(4):2046–2061. doi: 10.1167/iovs.11-8646

Figure 1.

Figure 1.

EN2 stimulated the most neurite outgrowth and the longest neurites from retinal explants of PN7 CB6F1 mice after 7 days in culture. (A) Histograph showing the mean (±SEM) number of neurites per explant (▪) or the mean (±SEM) of the 10 longest neurites per explant (█) grown in DMEM containing different growth supplements after 7 days in culture. Treatment with 10% EN2 produced significantly more neurites than treatment with any other 10% cell culture supplement (*P < 0.05, compared with 10% FBS, N2, and G5), and the neurites were significantly longer (**P < 0.01, compared with either 10% FBS, N2, and G5). (B–E) Photomicrographs showing PN7 CB6F1 mouse retinal explants treated with 10% FBS (B), 10% G5 (C), 10% N2 (D), or 10% EN2 (E) for 7 days then stained with phalloidin-Alexxa 488. Neurites were more numerous, longer, and more highly branched in EN2-treated explants than other treatment groups. Size bar = 15 μm.