Table 1.
Components of the Supplements Used in This Study
|
Supplement Name |
Source |
Components, μg/mL |
| N2 | Invitrogen/Gibco | Insulin (500) |
| Human transferrin (10,000) | ||
| Selenite (0.52) | ||
| Progesterone (0.63) | ||
| Putrascine (1611) | ||
| G5 | Invitrogen/Gibco | Insulin (500) |
| Human transferrin (5000) | ||
| Selenite (0.52) | ||
| Biotin (1) | ||
| Hydrocortisone (0.36) | ||
| bFGF (0.5) | ||
| EGF (1) | ||
| EN2 | Invitrogen/ Gibco (N2 only) | Insulin (500) |
| Human transferrin (10,000) | ||
| Selenite (0.52) | ||
| Progesterone (0.63) | ||
| Putrascine (1,611) | ||
| Biotin (1) | ||
| Hydrocortisone (0.36) | ||
| bFGF (0.5) | ||
| EGF (1) |
Commercially available supplements used are N2 (for neuronal cell cultures) and G5 (for glial cell cultures); both are available from Invitrogen/Gibco. Since N2 and G5 share some components, EN2 is made using commercial N2 with unique G5 components individually added.