Separation of pTS13 (P) and TS13 (N) in 140 mM borate, 70 mM SDS, pH 7.4 in a 50–μm diameter capillary (A) or with 140 mM borate, 150 mM SDS, 50 mM NaCl, pH 7.4 in a 30-μm diameter capillary (B). Reporter species were also separated from all fluorescent fragments (C), indicated by length in residues (e.g. fragment 1 is 6-FAM-Glu-COOH), as well as from the 6-FAM internal standard (F).