Table 2. Double reciprocal shift assay of a folA1 strain.
| Treatmenta | Initial Conditions |
Shift |
Percent with Two Nucleib | ||
|---|---|---|---|---|---|
| Temperature | Time, hr | Temperature | Time, hr | ||
| Downshifts | |||||
| A | 44° | 7 | No shift | 0 | 54 ± 1.7 |
| B | 44° | 7 | 28° | 3 | 55 ± 4.6 |
| C | 44° | 7 | 28° + 25 mM HUc | 3 | 46 ± 2.9 |
| Upshifts | |||||
| D | 28° + 25 mM HU | 7 | No shift | 0 | 0 |
| E | 28° + 25 mM HU | 7 | 28° | 3 | 27 ± 3.1 |
| F | 28° + 25 mM HU | 7 | 44° | 3 | 21 ± 2.4 |
HU, hydroxyurea.
a
One hundred germlings were counted for each experiment and the percentage having one nuclear division was determined.
b
Percentage of cells completing at least one nuclear division.
c
Before transferring the germlings to the treatment C (28° + HU), the germlings were incubated in the presence of 25 mM HU for 10 min at 44°.