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. Author manuscript; available in PMC: 2013 Jul 8.
Published in final edited form as: Methods Mol Biol. 2013;1005:77–93. doi: 10.1007/978-1-62703-386-2_7

Figure 1. Flow chart for fractionation and quantitative mass spectrometric analysis of chromatin.

Figure 1

Whole mouse hearts are homogenized, or adult cardiac myocytes are lysed, and the lysate enriched for nuclei. Each nuclei sample can either be separated into nucleoplasm and chromatin fractions or into an acid-extracted fraction that enriches for histones. Proteins from each are separated by one-dimensional SDS-PAGE, and the bands trypsin digested and run by LC/MS/MS.