Table 1. p53 Response elements in the PRODH gene.
| Name | Location (bp from TSS) | Mismatches HS 1 | Mismatches HS 2 | Spacer (bp) | Sequence | |
|---|---|---|---|---|---|---|
| 1 | -3.1 | Promoter, -3,158 | 1 | 2 | 5 | cGACTTGTCC TCAAT GAcCAcGCTC |
| 2 | -0.9 | Promoter, -917 | - | 1 in ¼ site, 3 in a HS | 7, 5 | CACC AGgCTCCACTATGGGCTTGTCTTCGTGtGACTTcTgT * |
| 3 | +1.7 | Int 2,+1,694 | 2 | 1 | - | GGGCAAGgaCGGGCATGCTa |
| 4 | +2.8 | Int 2,+2,816 | 2, 2, 2, 2, 3 in each of the 5 HS | 3,0,0,3 bp respectively | ttACAAGCCCTAGGctCATGCCTAGGCATGgTgGctCATGCCTGTAAttCTAGCaC ° | |
| 5 | +4.7 | Int 2,+4,727 | 3 | - | 3 | GtcCTTGTTg CCA GGGCATGCCT |
| 6 | +6.4 | Int 3,+6,453 | 1 | 2 | 8 | GGtCTTGCTCTGTTGCCCAGGCTAGagT |
| 7 | +6.8 | Int 3,+6,817 | - | 2 | - | AGGCTTGCCTcAGCATGTCg |
| 8 | +14.3 | Int 8,+14,269 | 2 | 1 | 2 | AGcCATGgTTCCAGcCAAGCCC |
| 9 | +15.8 | Int 9,+15,832 | - in ¼ site | 1 | 5 | TGTTTGTTAGAAGCATGTCa |
HS: half-site
*
same RE described in [38].
° Cluster formed by 5 half-sites: the 3 central half-sites contain a CATG core and are separated by 0 bp spacer, while the two external half-sites are separated by the central 3 half-sites by 3 bp spacers. All of the 5 half-sites contain at least 2 mismatches each, although they never involve the core of the consensus.
Bold name: REs selected for experimental validation
Bold/underlined: bases belonging to the indicated RE; italic: spacers; minuscule: mismatches within RE