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. 2013 Jul 8;8(7):e64830. doi: 10.1371/journal.pone.0064830

Table 3. Results of direct sequencing (red for sequences from the C. regab symbiont and blue for the L. chuni symbiont), and cloning*.

Site Specimen Species Marker gene
16S (98.4%) 23S (98.6%) aprA (95.1%) dsrB (92.8%) soxA(90.6%) coxI(97.4%) cbb3 (94%)
1- North 209 V2 to V5 C. regab (n = 13) x x x x x x x
211 Net5 V1 to V3 and V5
211 PC12 V1 to V5
209 V1* C. regab 100 (4) 100 (36) 100 (12) 100 (4) 100 (10) 100 (7) 100 (5)
211 Net5 V4* C. regab 100 (6) 100 (44) 100 (10) 100 (23) 100 (11) 100 (4) 100 (1)
2- Center 217 V4 and V5 C. regab (n = 2) x x x x x x x
217 V2* C. regab 100 (4) 100 (35) 100 (12) 100 (9) 100 (10) 88 (7); 12 (1) 100 (4)
217 V3* C. regab 91 (32); 9 (3) 100 (53) 86 (47); 14 (8) 100 (21) 100 (22) ND 100 (1)
3- Southwest 225 V2 and V4 C. regab (n = 2) x x x X x x x
225 V3* C. regab 100 (6) 100 (33) 100 (12) 100 (8) 100 (8) 100 (6) 100 (5)
225 V5* C. regab 100 (38) 100 (64) 70 (45); 30 (17) 100 (23) 40 (2); 60 (3) ND 100 (3)
225 V1* L. chuni 95 (19); 5 (1) 100 (58) 64 (33); 36 (19) 100 (19) 100 (8) 100 (7) 100 (15)
225 V6* L. chuni 100 (9) 100 (34) 100 (13) 100 (17) 100 (11) 86 (6); 14 (1) 100 (13)
225 V7* L. chuni 100 (15) 100 (46) 100 (12) 100 (9) 100 (8) 100 (17) 100 (11)

Percentage of divergence between the two symbiont sequences is given in parentheses in the header after each locus name. Given are details on the percentage (number) of sequences from C. regab and L. chuni symbiont (bold), and the number of clones sequenced in parentheses. ‘x’ indicates sequences obtained without cloning.