Table 3. Results of direct sequencing (red for sequences from the C. regab symbiont and blue for the L. chuni symbiont), and cloning*.
Site | Specimen | Species | Marker gene | ||||||
16S (98.4%) | 23S (98.6%) | aprA (95.1%) | dsrB (92.8%) | soxA(90.6%) | coxI(97.4%) | cbb3 (94%) | |||
1- North | 209 V2 to V5 | C. regab (n = 13) | x | x | x | x | x | x | x |
211 Net5 V1 to V3 and V5 | |||||||||
211 PC12 V1 to V5 | |||||||||
209 V1* | C. regab | 100 (4) | 100 (36) | 100 (12) | 100 (4) | 100 (10) | 100 (7) | 100 (5) | |
211 Net5 V4* | C. regab | 100 (6) | 100 (44) | 100 (10) | 100 (23) | 100 (11) | 100 (4) | 100 (1) | |
2- Center | 217 V4 and V5 | C. regab (n = 2) | x | x | x | x | x | x | x |
217 V2* | C. regab | 100 (4) | 100 (35) | 100 (12) | 100 (9) | 100 (10) | 88 (7); 12 (1) | 100 (4) | |
217 V3* | C. regab | 91 (32); 9 (3) | 100 (53) | 86 (47); 14 (8) | 100 (21) | 100 (22) | ND | 100 (1) | |
3- Southwest | 225 V2 and V4 | C. regab (n = 2) | x | x | x | X | x | x | x |
225 V3* | C. regab | 100 (6) | 100 (33) | 100 (12) | 100 (8) | 100 (8) | 100 (6) | 100 (5) | |
225 V5* | C. regab | 100 (38) | 100 (64) | 70 (45); 30 (17) | 100 (23) | 40 (2); 60 (3) | ND | 100 (3) | |
225 V1* | L. chuni | 95 (19); 5 (1) | 100 (58) | 64 (33); 36 (19) | 100 (19) | 100 (8) | 100 (7) | 100 (15) | |
225 V6* | L. chuni | 100 (9) | 100 (34) | 100 (13) | 100 (17) | 100 (11) | 86 (6); 14 (1) | 100 (13) | |
225 V7* | L. chuni | 100 (15) | 100 (46) | 100 (12) | 100 (9) | 100 (8) | 100 (17) | 100 (11) |
Percentage of divergence between the two symbiont sequences is given in parentheses in the header after each locus name. Given are details on the percentage (number) of sequences from C. regab and L. chuni symbiont (bold), and the number of clones sequenced in parentheses. ‘x’ indicates sequences obtained without cloning.