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. 2013 Jul 8;8(7):e67566. doi: 10.1371/journal.pone.0067566

Figure 3. mFCS analysis of the WT HSF1 and mutants in unstressed and stressed cells.

Figure 3

Comparison between the dynamics of WT HS1 and mutants analyzed by mFCS. Autocorrelation function (ACF) curves of HSF1 before (NHS) and after a one hour heat shock (HS). The ACF functions are normalized for molecule number. Abscissa is related to diffusion times. WT HSF1-eGFP (red), HSF1-ΔTRIM-eGFP (green), HSF1-K80Q-eGFP (blue) and HSF1-ΔDBD-eGFP (purple).