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. 2013 Jul 8;8(7):e67566. doi: 10.1371/journal.pone.0067566

Figure 12. Sizes of HSF1 complex in unstressed and heat shocked HeLa cells using glycerol gradient fractionation.

Figure 12

HSF1-eGFP and HSF1-K80Q-eGFP were estimated to 150 kD in NHS and superior to 669 kDa in HS. The complex size of HSF1- ΔTRIM-eGFP in NHS was estimated to 66–150 kDa in NHS and about 200 kDa in HS. The complex size of HSF1-ΔDBD-eGFP in NHS was estimated to 66–150 kDa in NHS and about 450 kDa in HS. TE: total extract before ultra-centrifugation. The fraction corresponding to standard proteins of masses 66, 200 and 669 kDa are indicated with arrows.