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. 2013 Jul 8;8(7):e68796. doi: 10.1371/journal.pone.0068796

Figure 5. Temperature increase worsened sodium azide/2-DG induced ER stress.

Figure 5

(A) Cells were subjected to treatments with sodium azide/2-DG, increased temperature or combination of sodium azide/2-DG plus increased temperature for a period of 30 minute or 1 hour. Control cells received no treatment. The cells were then collected and protein expressions were analyzed with western blot. β-actin was used as a loading control. (B) CHOP shRNA transfection significantly inhibited CHOP expression. SH-SY5Y cells were transfected with lentiviral particles encoding copGFP (mock) or CHOP shRNA. CHOP protein levels were analyzed by western blot. β-actin was used as a loading control. (C) CHOP shRNA transfection significantly reduced sodium azide/2-DG-induced cell death. Mock or CHOP shRNA transfected SH-SY5Y cells were treated with sodium azide/2-DG for 1 hour at 37°C and 41°C. Cell death was detected with trypan blue exclusion assay. * (p<0.05) and † (p<0.01) denote significant difference from the group received mock transfection.