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. 2013 Jul 8;8(7):e69659. doi: 10.1371/journal.pone.0069659

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© 2013 Pärn et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) SFV(Fluc) 4, a replication-competent RNA vector containing the Fluc marker in its nonstructural region; (B) pCMV-SFV(Fluc) 4, corresponding DNA/RNA layered vector; (C, D) Replicon vectors SFV(Fluc) 1-EGFP and pCMV-SFV(Fluc) 1-EGFP where the structural region required for virion formation was replaced by the EGFP sequence; (E) SFV(ZsGreen) 4, a replication competent virus containing the ZsGreen marker in its nonstructural region.

CMV-cytomegalovirus immediate early promoter. SG promoter of SFV is indicated by an arrow; plasmid backbones of DNA/RNA layered vectors (B, D) are not shown.