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. 2013 Jul 8;8(7):e69659. doi: 10.1371/journal.pone.0069659

Figure 8. Effects of recent transfection with anti-EGFP siRNA on SFV at 6 h post infection.

Figure 8

CHO cells were transfected with siRNA targeting EGFP mRNA using PF6 (B, C, D) or LF2000 (E, F, G) reagents. Transfected cells were infected with SFV(ZsGreen) 4 at an MOI of 0.1 at 2 h (B, E), 4 h (C, F) or 8 h (D, G) post transfection. All cells were fixed at 6 h post infection. The localization of nsP1 (red) of SFV was revealed using rabbit polyclonal antiserum as the primary detection reagent and Alexa 568 conjugated anti-rabbit antibody as the secondary antibody; ZsGreen was detected by its green fluorescence; and the co-localization of these signals is shown as yellow. Nuclei were counter-stained with DAPI (blue). Images were collected using a LSM 710 confocal microscope (Zeiss); scale represents 10 µm. Each panel shows a single optical slice from a characteristic infected cell, and the nuclei of several non-infected cells are also visible. Panel A shows a representative cell from the control experiment (no transfection, cell was fixed at 4 h after infection with SFV(ZsGreen) 4).