Skip to main content
. Author manuscript; available in PMC: 2014 Aug 9.
Published in final edited form as: Cancer Lett. 2013 May 9;336(1):222–230. doi: 10.1016/j.canlet.2013.05.002

Figure 1. IGFBP5 Deletional Mutant Constructs.

Figure 1

A. A schematic map shows the full-length and deletional mutants generated with the respective functional domains indicated: The N-terminal domain (N) contains an 80 amino acid region, the Linker domain (L) contains 89 amino acids and the C-terminal domain (C) contains 82 amino acids. The reported functions of each of the domains (N-terminal, Linker, and C-terminal) are indicated. Identical constructs with and without Flag tags were made. B. Expression of each construct was confirmed by RT-PCR and Western blot. C. The constructs were cloned into the AdEasy adenoviral expression system co-expressing RFP as an indicator of infectivity. Adenoviral transduction of each of the constructs is shown. Equal efficiency of adenoviral transduction for each construct was achieved by dose titration and direct visualization under fluorescence imaging.