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. Author manuscript; available in PMC: 2014 Aug 9.
Published in final edited form as: Cancer Lett. 2013 May 9;336(1):222–230. doi: 10.1016/j.canlet.2013.05.002

Figure 5. IGFBP5 deletional mutants’ effects on cell invasion.

Figure 5

A. Matrigel invasion assay: Full length (NLC), deletional mutants and RFP control adenoviruses were used to equally transduce 143B and MG63.2 osteosarcoma cells. The cells were plated on Matrigel coated polycarbonate membranes containing pores. Fetal calf serum was used as a chemoattractant. Representative images of 143B cells that migrated through the coated membrane are shown (MG63.2 data not shown). B. Random fields of migrated cells per treatment group were counted, averaged and depicted on the graph shown; the asterisk (*) indicates a significant difference in cell invasion compared to the RFP control (p < 0.01).