Figure 1.

MT organization and end distribution in the mitotic spindle of human U2OS cells. (A–D) Partial reconstructions of metaphase spindles in control and augmin RNAi cells. MTs are colored green. Chromosome-facing ends (all open) and pole-facing closed and open ends are marked with white, magenta, and yellow spheres, respectively. Surfaces of chromosomes are outlined in blue. The centriole pair and associated MTs are in gray. In three out of four augmin knockdown cells, abnormal centriolar MTs were observed (Fig. S2, D and E). The connection sites between mother and daughter MTs are red in D. Bar, 1 µm. (E) Distribution of putative minus ends near or away from the centriole. Combined data from three control or four augmin knockdown cells are displayed. Data on each cell are presented in Tables S1 and S2.