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. 2013 Jul 8;202(1):25–33. doi: 10.1083/jcb.201304031

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© 2013 Kamasaki et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Quantitative description of the end-links. (A) Comparison of the probability of end-link appearance between control and augmin RNAi–treated cells in the centriole-proximal area or the body of the spindle. Combined data from three control or four augmin knockdown cells are displayed. Data on each cell are presented in Tables S1 and S2. (B) Frequency of the end-link appearance on the closed ends near or away from the centriole. End-links were observed more frequently in the body of the spindle than around the centrioles. Combined data from three control cells are displayed in this graph. Data on each cell are presented in Table S1. (C) Length distributions of the rod-shaped end-links. (D–G) Angles between mother and daughter MTs (α; E), a rod-shaped end-link and a mother MT (β; F), and a rod-shaped end-link and a daughter MT (γ; G). Mother and daughter MTs were predominantly at small angles (E). The data presented in C and E–G are derived from tomographic volumes of four control cells. The sample numbers for analysis are presented for each cell in Tables 1 and S1.