Table 1.
Quantitative information of the analyzed cells
| Parameters | Control (four cells) | Augmin RNAi (four cells) |
| Number of MTs (/10 µm3) | 549 | 448 |
| Number of intraspindle MTs (/µm2)b | 68a | 32 |
| Number of closed ends (/10 µm3) | 142 | 138 |
| Number of pole-facing open ends (/10 µm3) (flared, blunt) | 66 (55, 11) | 76 (64, 12) |
| Number of ends near the centriole (/10 µm3) (closed, pole-facing open)c | 186a (135, 51) | 203 (135, 68) |
| Number of ends away from the centriole (/10 µm3) (closed, pole-facing open)d | 43a (23, 20) | 10 (2, 8) |
| Number of chromosome-facing open ends (/10 µm3) (flared, blunt) | 83 (81, 2) | 58 (52, 6) |
| Percentage of end-links (per total closed ends) (rod shaped, direct) | 8.8 (4.6, 4.2) | 1.1 (0.6, 0.5) |
| Number of end-links in total volume (rod shaped, direct) | 71 in 57 µm3 (37, 34) | 11 in 71 µm3 (6, 5) |
| Number of end-links/10 µm3 (rod shaped, direct) | 12 (6, 6) | 1.5 (0.8, 0.7) |
| Number of end-links/1-mm MT (rod shaped, direct) | 27 (14, 13) | 4.2 (2.3, 1.9) |
| Percentage of end-links near the centriole (/closed ends) (rod shaped, direct)c | 6.6a (3.1, 3.5) | ND |
| Percentage of end-links away from the centriole (/closed ends) (rod shaped, direct)d | 16.4a (10, 6.4) | ND |
| Length of rod-shaped end-link (nm, ±SD) | 29 ± 14 | ND |
| Angle α (°, ±SD); rod shaped | 14 ± 40 | ND |
| Angle α (°, ±SD); direct | 61 ± 47 | ND |
| Angle β (°, ±SD); rod shaped | 64 ± 17 | ND |
| Angle γ (°, ±SD); rod shaped | 131 ± 36 | ND |
a
Based on three cells for which the centriole center was detected in the tomographic volumes.
b
Numbers of MTs that pass through the plane 2.5 µm apart from the centriole center.
c
<1.5 µm from the centriole center.
d
≥1.5 µm from the centriole center.