Fig. 3.

The flow cytometric analysis of crocidolite fibers and MeT5A cells. (A) The cytogram of MeT5A cells without incubation with crocidolite. (B) The cytogram of crocidolite fibers alone revealed that most of these fibers displayed FSC values of less than 200. The amplification gain of the FSC photodiode detector was 8.50. (C) The cytogram without a threshold of forward scatter (FSC) included crocidolite fibers that had detached from the cell surface. To generate this cytogram, MeT5A cells at 70% confluence were incubated with 5 µg/cm² crocidolite for 2 h.