Table 2.
Influence of the incubation time on the crocidolite fiber uptake examined by 2 methods
Incubation time (h) | 1 | 4 | 24 |
---|---|---|---|
Mean SSC ratio (Treated/Control) | 1.277 | 1.435 | 2.07 |
†Cells without crocidolite (%) | 31.6 | 18.9 | 9.7 |
†Cells including less than 5 bundles of crocidolite fiber (%) | 45.8 | 42.8 | 21.8 |
†Cells including 5 or more bundles of crocidolite fiber/crocidolite in clumps (%) | 22.6 | 38.3 | 68.5 |
†Total counted cell numbers | 472 | 470 | 559 |
MeT5A cells were grown to 70% confluence (2 × 104 cells/cm2, 24 h before) and incubated with 5 µg/cm2 crocidolite. †Counted by cell block.