Fig. 3.

Secreted forms of the N-glycosylation reporters in C. albicans. Western analysis of secreted proteins from wild type C. albicans cells (CAI4) transformed with pACT-GR1, pACT-GR2, pACT-GR3 or the empty CIp10 vector (C, control) probed with the polyclonal anti-FLAG antibody. Extracts were either treated (+) or not treated (−) with endoglycosidase H before analysis and samples corresponding to equivalent culture volumes were run on the gels. The black arrow indicates the mature 23 kDa deglycosylated forms of the GR proteins. The asterisk highlights the band corresponding to endoglycosidase H itself.