Skip to main content
. 2013 Jul;56:107–115. doi: 10.1016/j.fgb.2013.03.009

Fig. 5.

Fig. 5

Effects of aberrant glycosylation upon GR2 processing in C. albicans. Western analyses of endoglycosidase H treated (+) and untreated (−) extracts from C. albicans mutants transformed with pACT1-GR2: wt, CAI4 cells transformed with pACT1-GR2; mnt, NGY112 (mnt1 mnt2) containing pACT1-GR2; och1, NGY205 containing pACT1-GR2; pmr1, NGY98 containing pACT1-GR2 (Table 1). Membranes were probed with the polyclonal anti-FLAG antibody. (A) Western analysis of intracellular protein extracts. (B) Western analysis of extracellular fraction. Samples corresponding to equivalent culture volumes: black arrow, the processed, deglycosylated 23 kDa form of GR2; white arrow, the unprocessed, deglycosylated 28 kDa pre-pro-form of GR2; asterisk, background band corresponding to endoglycosidase H.