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For human specimens, three (3) 3 × 5mm specimens were cut from the outer 2/3’s of the patella tendon and were either left untreated (control) or treated with genipin or cyanoacrylate glue. These specimens were then cultured with confluent ACL fibroblasts for 48 hours to observe any cytotoxic effects of genipin treatment. For both human and bovine specimens, a 2 × 2 mm biopsy (~100 mg) was cut from this tissue for determination of proteoglycan and collagen content.
